CTAB Extraction Buffer Solution, 250 mL -from Cepham Life Sciences

Cepham Life Sciences CTAB Extraction Buffer, pH 8, effectively removes polysaccharides and polyphenols with the use of Polyvinylpyrrolidone, a polyphenol binding agent. Cetyltrimethylammonium bromide (CTAB) is a cationic surfactant, commonly used in the preparation and purification of genomic DNA from different samples, particularly plants. Extracting DNA from plant tissues can be very challenging, primarily due to polysaccharides and polyphenols, two classes of plant biomolecules that vary widely between plant species and can inhibit the use of polymerase chain reaction (PCR) and other downstream applications. CTAB protocols typically require the homogenization of plant samples in CTAB Extraction Buffer prior to centrifugation to pellet the debris and polysaccharides. Since the DNA extract obtained with this method contains both DNA and RNA, RNA can be easily removed by the addition of RNase A. The DNA is precipitated and washed in organic solvents before re-dissolving in aqueous solution. For research only. Available in 125 mL and 250 mL sizes. Molecular Biology Grade. For research use only. Not for human or animal clinical/therapeutic use. Please follow all safety instructions.

• Contains 2% CTAB, 20 mM EDTA Na2·2H2O, 1.4 M NaCl, & 100 mM Tris
• pH8
• Ready-to-use solution provides savings in labor, raw materials, and waste disposal
• Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests
• Product is guaranteed to perform as per its SOP and is backed with a money back guarantee