Quantitative Elisa for 10 Lab Groups

Now with NEW substrate! Antibodies are highly specific in their recognition of antigens. This ELISA experiment demonstrates the quantitation of varying concentrations of viral antigens as detected by the intensity of the color reaction due to the accumulation of products. This laboratory activity meets the requirements in the BSCS Blue Biology curriculum.Group Size:For 10 lab groupsTime Required:Complete in 2 hoursKit Includes:Instructions, antigens, primary; secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipets.All You Need:Distilled or deionized water, 37º C incubation oven, automatic micropipettes with tips, laboratory glassware.Storage:Some Components Require Refrigerator Storage

• Kit Includes: Instructions, antigens, primary; secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipets.
• Prepare serial dilutions and perform ELISAs to determine the concentration of two antigens
• Explore the specificity of ELISAs by testing multiple antigen-antibody combinations
• Quantify results using imaging and graphing software
• Master the Enzyme-Linked Immunosorbent Assay (ELISA) with this advanced experiment